--- /dev/null
+#!/usr/bin/perl
+#use strict;
+# create the variables
+# keys is $gene_header, values is $seq
+my %seq_hash;
+my $gene_header;
+my $saeq;
+$file = "/lemma/Rajani/iprscan/Toly_new.fa";
+open(in_file,$file);
+
+while(<in_file>)
+{
+ my $line = $_;
+ chomp $line;
+
+
+ if($line =~ /^\>/)
+ {
+ if(defined($gene_header))
+ {
+ $seq_hash{$gene_header} = $seq;
+ }
+ $gene_header = $line;
+ $seq = "";
+ }
+ else
+ {
+ $seq = "$seq"."$line";
+ }
+ $seq_hash{$gene_header} = $seq;
+
+ }
+
+#foreach $key(%seq_hash)
+#{
+#print "Gene is $key and the sequence is $seq_hash{$gene_header} \n";
+#}
+
+while (($key, $value) = each(%seq_hash))
+{
+ # print $key."\n".$value."\n";
+
+ open(FILE1,">Toly.fa") || die("Cannot Open File");
+ print FILE1 "$key\n";
+ print FILE1 "$value";
+
+$astatus6 = system("/lemma/SignalP/targetp-1.1/targetp -P /lemma/Rajani/iprscan/Toly.fa >> targetp_4_Toly.out");
+
+}
+
+