From 7c6dd174c1d0c93618a4a473139ea7413ab3ae39 Mon Sep 17 00:00:00 2001 From: elserj Date: Tue, 2 Nov 2010 22:00:59 +0000 Subject: [PATCH] Fix line break problems svn path=/; revision=112 --- ..._growth_germplasm_S.lycopersicum_sgn.assoc | 45 ++----------------- 1 file changed, 3 insertions(+), 42 deletions(-) diff --git a/po-associations/po_growth_germplasm_S.lycopersicum_sgn.assoc b/po-associations/po_growth_germplasm_S.lycopersicum_sgn.assoc index a020474..f5d39fc 100644 --- a/po-associations/po_growth_germplasm_S.lycopersicum_sgn.assoc +++ b/po-associations/po_growth_germplasm_S.lycopersicum_sgn.assoc @@ -11207,48 +11207,9 @@ SGN_germplasm 9982 New Zealand Pear PO:0007010 SGN_ref:861 IDA G Source: sandh SGN_germplasm 9982 New Zealand Pear PO:0007042 SGN_ref:861 IDA G Source: sandhillpreservation germplasm taxon:4081 20090922 SGN SGN_germplasm 9982 New Zealand Pear PO:0001050 SGN_ref:861 IDA G Source: sandhillpreservation germplasm taxon:4081 20090922 SGN SGN_germplasm 9982 New Zealand Pear PO:0001083 SGN_ref:861 IDA G Source: sandhillpreservation germplasm taxon:4081 20090922 SGN -SGN_germplasm 9984 RNAi-tagl1 PO:0007010 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector -(kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 -cDNA sequences used in the haripin included 252 bp from the C domain -and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full- -length cDNA). The target sequences were PCR amplified from EST clone -cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev -with addition of the corresponding recombination sequences as defined -in the kit for site-specific recombination used (Gateway BP Clonase -enzyme mix Invitrogen). The resulting PCR product was gel purified and -cloned into pHELLSGATE 2 via homologous recombination using the kit -above. The resulting construct was sequence confirmed and transformed -into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain -ABA4404) as described previously (Vrebalov et al., 2002). -Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN -SGN_germplasm 9984 RNAi-tagl1 PO:0007038 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector -(kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 -cDNA sequences used in the haripin included 252 bp from the C domain -and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full- -length cDNA). The target sequences were PCR amplified from EST clone -cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev -with addition of the corresponding recombination sequences as defined -in the kit for site-specific recombination used (Gateway BP Clonase -enzyme mix Invitrogen). The resulting PCR product was gel purified and -cloned into pHELLSGATE 2 via homologous recombination using the kit -above. The resulting construct was sequence confirmed and transformed -into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain -ABA4404) as described previously (Vrebalov et al., 2002). -Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN -SGN_germplasm 9984 RNAi-tagl1 PO:0007042 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector -(kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 -cDNA sequences used in the haripin included 252 bp from the C domain -and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full- -length cDNA). The target sequences were PCR amplified from EST clone -cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev -with addition of the corresponding recombination sequences as defined -in the kit for site-specific recombination used (Gateway BP Clonase -enzyme mix Invitrogen). The resulting PCR product was gel purified and -cloned into pHELLSGATE 2 via homologous recombination using the kit -above. The resulting construct was sequence confirmed and transformed -into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain -ABA4404) as described previously (Vrebalov et al., 2002). -Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN +SGN_germplasm 9984 RNAi-tagl1 PO:0007010 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector (kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 cDNA sequences used in the haripin included 252 bp from the C domain and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full-length cDNA). The target sequences were PCR amplified from EST clone cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev with addition of the corresponding recombination sequences as defined in the kit for site-specific recombination used (Gateway BP Clonase enzyme mix Invitrogen). The resulting PCR product was gel purified and cloned into pHELLSGATE 2 via homologous recombination using the kit above. The resulting construct was sequence confirmed and transformed into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain ABA4404) as described previously (Vrebalov et al., 2002). Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN +SGN_germplasm 9984 RNAi-tagl1 PO:0007038 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector (kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 cDNA sequences used in the haripin included 252 bp from the C domain and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full-length cDNA). The target sequences were PCR amplified from EST clone cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev with addition of the corresponding recombination sequences as defined in the kit for site-specific recombination used (Gateway BP Clonase enzyme mix Invitrogen). The resulting PCR product was gel purified and cloned into pHELLSGATE 2 via homologous recombination using the kit above. The resulting construct was sequence confirmed and transformed into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain ABA4404) as described previously (Vrebalov et al., 2002). Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN +SGN_germplasm 9984 RNAi-tagl1 PO:0007042 PMID:19880793 IMP G The TAGL1 RNAi construct was made using the pHELLSGATE 2 vector (kindly provided by CSIRO, Plant Industry, Canberra, Australia). TAGL1 cDNA sequences used in the haripin included 252 bp from the C domain and the subsequent 172 bp of 39 UTR (i.e., bases 594 to 1017 of the full-length cDNA). The target sequences were PCR amplified from EST clone cLEG9L5 using gene-specific primers cLEG9L5For and cLEG9L5FRev with addition of the corresponding recombination sequences as defined in the kit for site-specific recombination used (Gateway BP Clonase enzyme mix Invitrogen). The resulting PCR product was gel purified and cloned into pHELLSGATE 2 via homologous recombination using the kit above. The resulting construct was sequence confirmed and transformed into tomato cv Ailsa Craig by Agrobacterium tumefaciens (strain ABA4404) as described previously (Vrebalov et al., 2002). Vrebalov et al., 2009. germplasm taxon:4081 20091103 SGN SGN_germplasm 9993 TAGL1-SRDX PO:0007038 PMID:19891701 IMP G A dominant repressor construct was created by generating a translational fusion between the EAR repression domain (SRDX Hiratsu et al., 2003) and the 3' end of the TAGL1 cDNA, introducing TAGL1–SRDX into pAA100 (using NcoI and SacI sites) containing the E8 promoter (the 35S CaMV in pAA100 was replaced previously by the E8 promoter through BamHI and NcoI cloning), and transfer of the E8::TAGL1-SRDX cassette to pBIN-PLUS (using PacI and AscI sites). Constructs were transformed into cv. MicroTom germplasm taxon:4081 20091211 SGN SGN_germplasm 9993 TAGL1-SRDX PO:0007042 PMID:19891701 IMP G A dominant repressor construct was created by generating a translational fusion between the EAR repression domain (SRDX Hiratsu et al., 2003) and the 3' end of the TAGL1 cDNA, introducing TAGL1–SRDX into pAA100 (using NcoI and SacI sites) containing the E8 promoter (the 35S CaMV in pAA100 was replaced previously by the E8 promoter through BamHI and NcoI cloning), and transfer of the E8::TAGL1-SRDX cassette to pBIN-PLUS (using PacI and AscI sites). Constructs were transformed into cv. MicroTom germplasm taxon:4081 20091211 SGN SGN_germplasm 9993 TAGL1-SRDX PO:0007010 PMID:19891701 IMP G A dominant repressor construct was created by generating a translational fusion between the EAR repression domain (SRDX Hiratsu et al., 2003) and the 3' end of the TAGL1 cDNA, introducing TAGL1–SRDX into pAA100 (using NcoI and SacI sites) containing the E8 promoter (the 35S CaMV in pAA100 was replaced previously by the E8 promoter through BamHI and NcoI cloning), and transfer of the E8::TAGL1-SRDX cassette to pBIN-PLUS (using PacI and AscI sites). Constructs were transformed into cv. MicroTom germplasm taxon:4081 20091211 SGN -- 2.34.1